Journal: Aging Brain
Article Title: Aging changes the expression of adenosine receptors, insulin-like growth factor 1 (IGF1), and hypoxia-inducible factor 1α (HIF1α) in hypothalamic astrocyte cultures
doi: 10.1016/j.nbas.2023.100104
Figure Lengend Snippet: Aging decreases expression and levels of IGF1 and HIF1α in hypothalamic astrocyte cultures. RT-PCR quantification of mRNA expression of IGF1R (A) and HIF1α (C), extracellular levels of IGF1 (B) and intracellular content of HIF1α (D). For RT-PCR and ELISA measurements, the data represent the mean ± SEM of triplicate samples from 6 independent cultures (each culture derived from 3 hypothalamus). The data were statically analyzed by one-way ANOVA followed by Tukey’s test. a refers to statistically significant differences from newborn rats (2 days old); b refers to statistically significant differences from 90 days old rats.
Article Snippet: IGF1: Invitrogen (#ERIGF1), analytical sensitivity: 30.0 pg/ml, detection range: 30.72 – 7500 pg/ml; HIF1α: Elabscience (#E-EL-R0513), analytical sensitivity: 4.7 pg/ml, detection range: 7.81 – 500 pg/ml.
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Derivative Assay